Skip to main content

Table 1 Validation (qPCR) of genes involved in bile acid metabolism after S. Typhimurium infection.

From: Transcriptional analysis of porcine intestinal mucosa infected with Salmonella Typhimurium revealed a massive inflammatory response and disruption of bile acid absorption in ileum

Gene

Day 2

Day 6

qPCR

Array

qPCR

Array

APOA1

−50.00**

−33.32**

−1.76

–

C-FOS

7.00*

–

1.87

–

C-JUN

1.87

–

1.33

–

FABP2

−50.00*

−48.16***

−2.58

–

FABP6

−200.00**

−56.24*

−2.28

–

IL-1B

30.22**

3.4*

2.92

–

IL-6

1.69

–

−1.91

–

NR1H4 (FXR)

−3.73

−4.68*

1.34

2.02*

PPARG

1.70

1.88**

1.39

–

RXRG

−4.09*

−2.92**

1.56

–

SLC10A2 (ASBT)

−1.92*

–

1.38

 

STAT3

1.64

 

1.57

−1.6*

TLR2

2.99*

1.57*

3.43

2.41*

TLR4

10.44*

1.88*

2.25

3.52*

TLR9

−1.79

 

−1.29

−1.92*

TNF-α

8.68*

1.59*

−1.26

–

  1. All values are expressed in fold change (FC) compared to controls. Asterisks indicate statistically significant values (compared to controls).
  2. * P < 0.05; ** P < 0.01; *** P < 0.001.