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Figure 1 | Veterinary Research

Figure 1

From: Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages

Figure 1

Streptococcus uberis strains from clinical or subclinical cases of mastitis did not activate immune functions of pbMEC. Induction of gene expression (ordinate) of TNF, CXCL8 and CYP1A1 after challenging for 0, 1, 3 and 24 h (abscissa) with 30 µg/mL of 14 different heat-killed S. uberis strains isolated from clinical (A) or subclinical (B) cases of mastitis compared against E. coli 1303. Values are RT-qPCR measurements of the respective mRNA species, normalized against CLIC1 and expressed as multiple of the value measured from the unstimulated control. Values are from a single experiment, assayed in duplicate. Only E. coli 1303 elicited a significant induction (compared to control, p < 0.05) of TNF and CXCL8. All preparations strongly induced the expression of CYP1A1.

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