Table 1 Primers covering gag, pol, LTR and env regions, were used for the SRLV detection by PCR
From: Post-entry blockade of small ruminant lentiviruses by wild ruminants
PCR assay | Primers | Sequence 5′–3′ | References |
|---|---|---|---|
Gag nested PCR | GAG F1 | TGG TGA RKC TAG MTA GAG ACA TGG | [64] |
GAG F2 | CAA ACW GTR GCA ATG CAG CAT GG | ||
POL R1 | CAT AGG RGG HGC GGA CGG CAS CA | ||
POL R2 | GCG GAC GGC ASC ACA CG | ||
Pol nested PCR | P28 | CAT GAA GAG GGG ACA AAT CAG CA | [18] |
P34 | TAC ATT GGG TGC CTG GAC ATA A | ||
P32 | TAC CTG DGT TGG TCC YWG CCA CT | ||
P33 | CTT CCC AVA GTA CCT GDG TTG GTC | ||
P29 | GGT GCC TGG ACA TAA AGG GAT TC | ||
P35 | GCC ACT CTC CTG RAT GTC CTC T | ||
Gag-Can nested PCR | CAEV F0 | AAC TGA AAC TTC GGG GAC GCC TG | [5] |
CAEV F1 | AAG GTA AGT GAC TCT GCT GTA CGC | ||
CAEV F2 | TGG TGA GTC TAG ATA GAG ACA TGG | ||
CAEV R0 | GTTATCTCGTCCTAATACTTCTACTGG | ||
CAEV R1 | TTT TTC TCC TTC TAC TAT TCC YCC | ||
CAEV R2 | GGA CGG CAC CAC ACG TAK CCC | ||
MVV F0 | AAG TAA GGT AAG AGA GAC ACC TAC TGG | ||
MVV F1 | TAG ATA GAG ACA TGG CGA AGC AAG CTC | ||
MVV F2 | GAC AGA AGG GAA CTG TCT ATG GGC | ||
MVV R0 | GGT GGT GCT TCT GTT ACA ACA TAG G | ||
MVV R1 | GGA CGG CAC CAC ACG TGG | ||
MVV R2 | CCC CTC CTG YTG TTT CCC TG | ||
BIV nested PCR | POL3 | ACA ACG GGC CGT GCT TTA CTG | |
POL4 | CCT CTT CCT CTA TTA CTG CTG C | ||
POL5 | GAR AAT CTA TGT AAG TAT CTG GG | Unpublished data (Nadin-Davis) | |
POL6 | CTG TTY CTT ACG TAA CAC CAC T | ||
P09 | CAC TGG ACG AGA TGA GGT AGT | [67] | |
P06 | TGG TAG TCT GAT AAA TGG CA | ||
P04 | CAG GCT CTT AAG GAA ATT GT | ||
P11 | CCA TCC TTG TGG TAG AAC ATT | ||
Craft-Oslo | Craft-Fw | TGA CAG AAG GAA ATT GTY TRT GG | |
Oslo-Rv | GGC ATC ATG GCT AAT ACT TCT AA | [68] | |
LTR PCR | LTR-Fw | TGA CAC AGC AAA TGT AAC CGC AAG | [69] |
LTR-Rv | CCA CGT TGG GCG CCA GCT GCG AGA | ||
CO qPCR | 612-F | GAA CTC AGC CAC AAG AGG AAG AA | [70] |
613-R | CCT GCG GCA GCT ACT ATT GC | ||
614 Probe | FAM AACTAGCATAATGACCAAGCCAACGCCTCT BHQ-1 | ||
A4 qPCR | Fs7631/Fg6221 | ACA AAC TGG ACC ACC ATG CA | [21] |
Rg6221 | CTA GTG TTC CAT TTC CTG TTC TGT TTA | ||
Probe g6221 | FAM GGC AAC TGT TCW CAG TGT GTT AAT GYA ACG BHQ-1 | ||
A4-SU PCR | SU5-1F | GTA GAT GTG TAC AAA GAC CAG | [21] |
SU5-1R | CTG CCT CTA ACA CTT GCT GC | ||
CO-SU PCR | B1-SU5-1R | TGC CTC TAA CAC ATC CTG CTG TGC | [70] |
B1-SU5-1F | GGT GGA ACA TAT GAC AGG AG |