Expression of cytokine and apoptosis-related genes in bovine peripheral blood mononuclear cells stimulated with Brucella abortus recombinant proteins

Table 2 Primer sets used for real-time PCR

Gene	Primer sequence (5′–3′)	Annealing temperature (°C)	Product size (bp)	Reference
iNOS	F: AGCGGAGTGACTTTCCAAGA	55	97	[40]
iNOS	R: TTTTGGGGTTCATGATGGAT	55	97	[40]
IL-1β	F: ACCTTCATTGCCCAGGTTTCT	55	120	[40]
IL-1β	R: TGTTTGGGGTCATCAGCCTCAA	55	120	[40]
IL-4	F: CAAAGAACACAACTAAGAAG	55	181	[40]
IL-4	R: AGGTCTTTCAGCGTACTTGT	55	181	[40]
IL-6	F: TCCAGAATGAGTATGAGG	55	236	[40]
IL-6	R: CATCCGAATAGCTCTCAG	55	236	[40]
IL-12p40	F: AACCTGCAACTGAGACCATT	55	186	[40]
IL-12p40	R: ATCCTTGTGGCATGTGACTT	55	186	[40]
IFN-γ	F: ATAACCAGGTCATTCAAAGG	55	218	[40]
IFN-γ	R: ATTCTGACTTCTCTTCCGCT	55	218	[40]
TNF-α	F: TAACAAGCCAGTAGCCCACG	55	277	[40]
TNF-α	R: GCAAGGGCTCTTGATGGCAGA	55	277	[40]
Bax	F: TCTCCCCGAGAGGTCTTTTT	55	151	[41]
Bax	R: TGATGGTCCTGATCAACTCG	55	151	[41]
Bcl-2	F: ATGTGTGTGGAGAGCGTCAA	55	146	[41]
Bcl-2	R: CTAGGGCCATACAGCTCCAC	55	146	[41]
TLR4	F: TGACATCTTCACAGAACTGACTA	55	164	This study
TLR4	R: GGAGTGGTTCATAAAGAAATGTA	55	164	This study
β-actin	F: CGCACCACTGGCATTGTCAT	60	227	[42]
β-actin	R: TCCAAGGCGACGTAGCAGAG	60	227	[42]

ISSN: 1297-9716