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Figure 3 | Veterinary Research

Figure 3

From: Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs

Figure 3

Characterization of VRP-mediated expression of the GP3 ectodomain. A Immunofluorescence analysis of MARC-145 cells 6 h after infection with VSV*ΔG(GP3ecto-Myc) or with the VSV*ΔG control. Expression of GP3ecto is detected with a rabbit anti-Myc serum (top panels) and with the anti-GP3 mAb VII2D (bottom panels). B Western blot analysis of lysates (ly) and supernatants (sn) from cells infected with VSV*ΔG(GP3ecto-Myc) separated by SDS-PAGE under non-reducing (-βME) conditions, using the anti-Myc serum for GP3ecto-Myc detection (arrowhead). Protein molecular weight (kDa) standards are indicated.

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Veterinary Research

ISSN: 1297-9716