Per os infectivity of white spot syndrome virus (WSSV) in white-legged shrimp (Litopenaeus vannamei) and role of peritrophic membrane

Table 1 Determination of virus titers of a WSSV stock in P. vannamei by different inoculations.

Experiment	Inoculation route	Dilution on homogenate of tissue	No. of shrimp	Mortality at different time points (hpi)									Infection by IIF	Virus titer
Experiment	Inoculation route	Dilution on homogenate of tissue	No. of shrimp	24	36	48	60	72	84	96	120	Total	Infection by IIF	Virus titer
1	Intramuscular	10⁻⁷	5		1	1	1		1			4/5	4/5	10^8.8SID₅₀/g
		10⁻⁸	5				1					1/5	1/5
		10⁻⁹	5									0/5	0/5
	Peroral	10⁰	5			1	1					2/5	2/5	10^1.1SID₅₀/g
		10⁻¹	5									0/5	0/5
		10⁻²	5									0/5	0/5
		10⁻³	5									0/5	0/5
	Feed	0.5	5									0/5	0/5	10^0.6SID₅₀/g
		5	5		1							1/5	1/5
		50	5									0/5	0/5
		100	5		1							1/5	1/5
		250	5		1	1						2/5	2/5
		500	5					3				3/5	3/5
2	Intramuscular	10⁻⁶	5		1	3		1				5/5	5/5	10^8.8SID₅₀/g
		10⁻⁷	5			1	2	1				4/5	4/5
		10⁻⁸	5				1					1/5	1/5
		10⁻⁹	5									0/5	0/5
	Peroral	10⁰	5				2	1				3/5	3/5	10^1.5SID₅₀/g
		10⁻¹	5									0/5	0/5
		10⁻²	5									0/5	0/5
		10⁻³	5									0/5	0/5
	Feed	0.5	5									0/5	0/5	10^0.8SID₅₀/g
		5	5									0/5	0/5
		50	5				1					1/5	1/5
		100	5				2					2/5	2/5
		250	5		2	1						3/5	3/5
		500	5		1		2	1				4/5	4/5
3	Intramuscular	10⁻⁶	5		2	3						5/5	5/5	10^8.7SID₅₀/g
		10⁻⁷	5			2	2					4/5	4/5
		10⁻⁸	5									0/5	0/5
		10⁻⁹	5									0/5	0/5
	Peroral	10⁰	5				1	1				2/5	2/5	10^1.1SID₅₀/g
		10⁻¹	5									0/5	0/5
		10⁻²	5									0/5	0/5
		10⁻³	5									0/5	0/5
	Feed	0.5	5									0/5	0/5	10^0.8SID₅₀/g
		5	5									0/5	0/5
		50	5					1				1/5	1/5
		100	5					2				2/5	2/5
		250	5				1	1				2/5	2/5
		500	5				2	1	1			4/5	4/5

ISSN: 1297-9716