Figure 2

Histopathologic changes and the location of virus antigen in the magnum and uterus of hens infected with H9N2 subtype AIV. Histopathologic changes of the magnum and uterus in control and AIV-infected groups on day 5 post inoculation was observed by hematoxylin and eosin (H&E) staining (400×). (a) degeneration and necrocytosis of mucosal epithelial cell layer; (b) glands edema or dissolved; (c) infiltration of lymphocytes. Nuclei of the tissue sections from control and infected hens were stained with Hoechst 33342: red colour fluorescence indicates the positive staining of virus NP proteins. Scale bar = 50 µm.