Figure 5

Investigation and characterization of bovine MEFV. A Average MEFV mRNA levels detected relative to the relevant uninfected sample. Error bars illustrate the standard error of six biological replicates. Asterisk denotes that the variation between live and inactivated S. Typhimurium stimulation was statistically different by t test (P < 0.05). B Schematic diagram of human, murine and bovine MEFV proteins illustrating the major protein domains using the web-based SMART tool which identifies protein motifs and architecture [30]. Three splice variants of bovine MEFV were detected; SP1, SP2 and SP3, which are predicted to encode for two protein isoforms. All the proteins contain an N-terminal Pyrin domain as well as B box (BB) and coiled-coil (C) domains. However, only human MEFV encodes a PRY/SPRY (B30.2) domain.