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Table 4 Summary of qRT-PCR results quantifying the expression of genes associated with the three major GO terms investigated in DC and Mø infected with live Salmonella Typhimurium and stimulated with paraformaldehyde-inactivated S. Typhimurium

From: Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells

Gene symbol

GO term

Average fold changes

DC_L

DC_D

Mø_L

Mø_D

ATF3

RT

9.8 ± 1.1a, b

6.1 ± 0.5a, b

8.5 ± 0.6a

6.7 ± 1.2a

BIRC3

CD

64.0 ± 15.1a

51.0 ± 8.7a

81.3 ± 17.1a

60.0 ± 9.0a

Bt.17514 (TNFAIP3)

IR, CD

53.1 ± 9.8a

43.1 ± 5.7a

51.8 ± 12.7a

59.2 ± 14.3a

Bt.19462 (CCL5)

IR, CD

641.0 ± 184.5a, b

403.3 ± 90.9a, b

443.5 ± 133.6a, b

270.9 ± 81.0a, b

CSF2

IR, CD

182.0 ± 21.1a, b

119.3 ± 26.3a, b

257.2 ± 98.5a

198.2 ± 78.9a

CSF3

IR

308.9 ± 92.0a

558.2 ± 243.3a

633.8 ± 135.1a

548.3 ± 93.2a

CXCL2

IR

260.6 ± 38.2a, b

147.1 ± 24.4a, b

521.6 ± 159.7a, b

209.9 ± 46.0a, b

ETS1

RT, CD

0.9 ± 0.5

0.5 ± 0.5

0.2 ± 0.6

0.0 ± 0.6

FOSB

RT

3.8 ± 0.9a, b

1.5 ± 0.9b

4.2 ± 1.1a, b

1.6 ± 0.6a, b

HHEX

RT

−6.0 ± 1.3a, b

−9.9 ± 2.4a, b

−8.4 ± 1.0a

−9.8 ± 2.5a

ID1

RT, CD

71.2 ± 25.6a

102.1 ± 39.2a

103.0 ± 53.3a, b

77.2 ± 42.7a, b

ID2

RT

3.1 ± 0.6a, b 

1.5 ± 0.6b

2.6 ± 0.1a

2.2 ± 0.3a

ID3

RT, CD

−2.2 ± 0.3a

−2.4 ± 0.2a

−1.9 ± 0.2a

−2.4 ± 0.3a

IFNG

IR, CD

589.3 ± 190.5a, b

25.1 ± 12.0a, b

22.0 ± 9.2a, b

2.1 ± 0.8b

IL1B

IR, CD

1850.1 ± 415.0a

1353.8 ± 333.5a

3248.0 ± 1069.1a

2048.6 ± 611.3a

IL6

IR, CD

79.5 ± 20.7a

77.9 ± 31.2a

171.1 ± 89.3a

97.7 ± 37.9a

IL10

IR, CD

69.5 ± 20.4a

107.3 ± 31.8a

125.7 ± 44.6a

112.2 ± 18.6a

IL12B

IR, CD

17 044.3 ± 16 476.7a, b

34 218.1 ± 32 873.8a, b

2591.0 ± 1834.2a

3340.4 ± 1966.5a

ISG15

IR

304.8 ± 57.8a, b

165.7 ± 28.0a, b

120.0 ± 48.2a, b

46.8 ± 19.0a, b

JUNB

RT

15.2 ± 4.1a, b

7.4 ± 0.9a, b

12.2 ± 1.0a, b

8.7 ± 1.0a, b

KLF5

RT

9.0 ± 0.8a

9.4 ± 1.3a

8.7 ± 1.5a

9.1 ± 1.4a

KLF13

RT

−2.9 ± 0.5a

−2.7 ± 0.3a

−3.1 ± 0.2a

−2.5 ± 0.3a

LIG4

RT, CD

2.9 ± 0.3a

4.0 ± 0.5a

3.2 ± 0.3a, b

4.9 ± 0.5a, b

MEFV

IR

82.0 ± 27.7a

111.6 ± 15.8a

76.5 ± 14.3a, b

140.1 ± 16.2a, b

MYC

RT, CD

−0.5 ± 0.8b

−2.3 ± 0.5a, b

−0.9 ± 0.6

−1.7 ± 0.2a

NR4A2

RT, CD

4.4 ± 0.8a, b

1.1 ± 0.5b

2.6 ± 0.2a

1.9 ± 0.3a

PLEKHF1

CD

−5.2 ± 0.7a

−4.1 ± 0.6a

−3.8 ± 0.6a

−3.2 ± 0.3a

TNF

IR, CD

353.7 ± 104.3a

368.6 ± 58.0a

551.6 ± 282.1a

452.5 ± 171.5a

TNFAIP6

IR

683.3 ± 301.9a

927.7 ± 377.9a

9115.6 ± 5564.9a

9148.4 ± 6115.0a

TXNIP

IR, RT, CD

2.2 ± 0.8a, b

1.8 ± 0.7b

3.0 ± 0.3a, b

0.9 ± 0.5b

YWHAB

RT, CD

−1.3 ± 0.1a

−0.8 ± 0.4

−0.4 ± 0.5

−1.1 ± 0.0a

  1. The results are expressed as the average fold change in mRNA levels in DC treated with live (DC_L) and inactivated (DC_D) S. Typhimurium and Mø treated with live (Mø _L) and inactivated (Mø _D) S. Typhimurium compared to the uninfected DC or Mø ± standard errors.
  2. aIndicates when the mean fold change values were significantly different from resting cells by t test corrected for multiple testing (Benjamini–Hochberg) (P < 0.05).
  3. bDenotes when the mean fold change measured in DC and/or Mø is significantly different in the response to live and inactivated Salmonella by t test corrected for multiple testing (Benjamini–Hochberg) (P < 0.05). The GO term column indicates which of the three major GO terms the gene is associated with; inflammatory response (IR), regulation of transcription (RT) and regulation of programmed cell death (CD).