Figure 4
From: Regulation of tracheal antimicrobial peptide gene expression in airway epithelial cells of cattle
Effect of dexamethasone on LPS-, Pam3CSK4- and IL-17A-induced up-regulation of TAP mRNA. Bovine tracheal epithelial cells were grown in the presence or absence of 10−6 M dexamethasone for 24 h. LPS (0.1 μg/mL), Pam3CSK4 (1 μg/mL) or IL-17A (316 ng/mL) were added for 16 h. Each of these agonists significantly upregulated TAP gene expression relative to the reference gene GAPDH (P < 0.001, two-way ANOVA of log-transformed data, Tukey’s post-test). The LPS-, Pam3CSK4- and IL-17A-induced upregulation were each abrogated by pre-treatment with dexamethasone (P < 0.001, two-way ANOVA of log-transformed data, Tukey’s post-test, n = 3 per group). Repetition of the experiment using cells from a different animal yielded similar findings.