Figure 2
Dynamics of body temperature and PRRSV load in the plasma and BAL fluid of pigs. (A) Rectal temperature of pigs was recorded at the indicated dpv and dpc. PRRSV RNA copies and replicating infectious virus load in the plasma (B–D) and BAL fluid (E, F) were quantified by qRT-PCR and indirect immunofluorescence assay. The numbers above the bar in the graphs (E, F) represent the ratio of PRRSV positive pigs to the total in each group. Each data point or bar in the graph represents the average value from 4 or 5 pigs ± SEM. Asterisk marked in panel C indicates the virus titers in MLV-IM, MLV-CPG IM and MLV-IM Groups were significantly reduced compared to mock (PBS) challenged Group (P < 0.01) and MLV-CPG IN Group (P < 0.05) at 7 dpc. In panel D asterisks (*P < 0.05 and **P < 0.01) indicate statistical significant difference between the marked two pig Groups at 7 dpc.