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Figure 2 | Veterinary Research

Figure 2

From: Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation

Figure 2

SDS-PAGE and Western immunoblotting analysis of the rGroEL protein expressed in E. coli BL21. Lane M, protein molecular mass marker; lane 1, Western blot analysis of puried rGroEL protein using pre-immune mini-pig sera as the negative control; lane 2, Western blot analysis of puried rGroEL protein using mini-pig convalescent sera; lane 3, the elution of the purified rGroEL were separated on an SDS-PAGE and stained with coomassie; lane 4, rGroEL protein in E. coli BL21induced with 1 mM IPTG for 4 h; lane 5, rGroEL protein without IPTG; lane 6, empty expression vector pET28a (+).

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