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Figure 1 | Veterinary Research

Figure 1

From: Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Figure 1

Expression kinetics of interferons mRNA. A Interferons expression in fold change. The ∆∆Ct \(\left[ {\Delta \Delta {\text{Ct}} = \Delta {\text{Ct}}_{\text{WNV}} - \Delta {\text{Ct}}_{\text{mock}} } \right]\) values were calculated by subtracting the ∆Ct of genes in mock-inoculated PBMCs. The bar graph showed the expression of genes in WNV-infected PBMCs over mock-inoculated PBMCs (fold change: the normalised expression value of a gene in WNV-stimulated cells/the normalised expression value of a gene in mock-inoculated cells). Bars without common superscripts (A, B) denote statistical significant difference among time points (P < 0.05). B–D Relative expression of interferons mRNA, accounting for the effects of culture conditions on gene transcription in WNV- and mock-inoculated equine PBMCs. To compare the normalised expression of interferon genes from PBMCs harvested at each time point to their respective expression levels before either WNV- or mock- inoculation, the ∆∆Ct values were calculated by subtracting ∆Ct of genes in fresh-isolated PBMCs from the ∆Ct of genes in WNV- or mock- inoculated PBMCs at each time-point (for WNV-stimulated PBMCs, \(\Delta \Delta {\text{Ct}}_{\text{WNV}} = \Delta {\text{Ct}}_{\text{WNV}} - \Delta {\text{Ct}}_{\text{fresh}}\); and for mock-inoculated PBMCs, \(\Delta \Delta {\text{Ct}}_{\text{mock}} = \Delta {\text{Ct}}_{\text{mock}} - \Delta {\text{Ct}}_{\text{fresh}}\)). *P < 0.05.

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