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Figure 7 | Veterinary Research

Figure 7

From: Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Figure 7

mRNA quantification and viral antigen detection in tissues from WNV-infected horses. A, B Quantification of selected genes transcripts in tissues from experimentally infected horses. A lymphoid tissues (draining lymph node, spleen) and (B) brain in fold change. To compare the gene expression between WNVNSW2011-challenged and uninfected horses, fold change was calculated (fold change: the normalised expression value of a gene in WNV-challenged horsecells/the normalised expression value of a gene in uninfected horse cells). C, D Immunolabeling of viral antigen and inflammatory cells in experimentally infected horse tissues. No viral antigen was detected (A) despite perivascular (arrow) and parenchymal infiltration of leukocytes, including CD3 + cells (D, red stain, arrow) in experimentally infected equine brain (Medulla oblongata). The results from horse no. 2 are presented and are representative of all three horses. Antigen of interest on both sections were visualised with AEC substrate (red product) and sections were counterstained with Meyer’s haematoxylin (Magnification: ×20).

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