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Table 1 Bacterial strains and plasmids used in this study

From: A live attenuated Salmonella Enteritidis secreting detoxified heat labile toxin enhances mucosal immunity and confers protection against wild-type challenge in chickens

Strain/plasmid

Description

Reference

Salmonella Enteritidis

 JOL1182

Wild type isolate from chicken, challenge strain

Lab stock

 JOL860

Wild type isolate from chicken for antigen preparations

Lab stock

 JOL1087

ΔlonΔcpxRΔasd, used as base vaccine strain

[21]

 JOL1641

JOL1087 containing pJHL65-dmLT

This study

Plasmids

 pJHL65

asd + vector, pBR ori, b-lactamase signal sequence-based periplasmic secretion plasmid, 6xHis, high copy number

[34]

 pJHL65-dmLT

pJHL65 containing dmLT constitutively express under Ptrc promoter, secreted under bla secretory system

This study