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Table 2 Immunization of hens with the Salmonella Enteritidis strains and challenge

From: A live attenuated Salmonella Enteritidis secreting detoxified heat labile toxin enhances mucosal immunity and confers protection against wild-type challenge in chickens

Group (n = 10)

Constructed strain

Route

Dosagea

Challengeb

A

PBS control

Oral

200 µL sterile PBS

 
    

JOL1182

1 × 109 cells/200 µL PBS

B

JOL1087

Oral

1 × 108

 

Intramuscular

1 × 107

 

C

JOL1641

Oral

1 × 108

 

Intramuscular

1 × 107

 
  1. aDose for immunization expressed as number of Salmonella Enteritidis cells/200 µL of PBS.
  2. bChickens were challenged at the 9th week of age via oral route. Chickens were euthanized at 7 and 14 days post-challenge.