Table 2 Primers, position, product size and annealing conditions for detection of hemolysis related genes tlyA, tlyB, tlyC, hemolysin III, hemolysin activation protein and hemolysin III channel protein
From: Variation in hemolytic activity of Brachyspira hyodysenteriae strains from pigs
Target gene: primer names | Nucleotide sequence (5′ → 3′) | Position (NC_012225.1) | Product size (bp) | Temperature annealing (°C) |
|---|---|---|---|---|
tlyA: hemolysin A | ||||
tlyAS1Fo | GGTATTGGAGATGAATATAC | 267 034–267 054 | 956 | 58 |
tlyAS1Re | TGATGTAGAAGGCTTCTATA | 267 969–267 989 |  |  |
tlyB: hemolysin B | ||||
tlyBS3Fo | GGAGTGGAGAGAAAGTATTA | 1 414 613–1 414 633 | 974 | 57 |
tlyBS3Re | TGCTGTAAGCAGACTTATAG | 1 415 566–1 415 586 |  |  |
tlyBS4Fo | AGCTGTCCTTCTTCAAGTAC | 1 415 413–1 415 433 | 390 | 63 |
tlyBS4Re | AGTCGTAGGACAGAAAGAAG | 1 415 782–1 415 802 |  |  |
tlyBS2Fo | CCCTCTTCATAACCAACATA | 1 415 533–1 415 553 | 1062 | 65 |
tlyBS2Re | AGGGACTTGCTGAAAAGATA | 1 416 653–1 416 673 |  |  |
tlyBS1Fo | TTGTACCAGCAACAACTGAA | 1 416 575–1 416 595 | 1082 | 54 |
tlyBS1Re | AGCTCTATCTACAGCAATAC | 1 417 635–1 417 655 |  |  |
tlyC: hemolysin C | ||||
tlyCFo | TTACGAATGCCTGCTATTTG | 1 644 915–1 644 935 | 1131 | 50 |
tlyCRe | CTATTTTTAGGCGAGGCTTT | 1 646 025–1 646 045 |  |  |
BHWA1_RS02195: hemolysin III | ||||
HlysCBSFo | GGAAAAAGGGATCCTGGAAC | 704 725–704 745 | 1570 | 54 |
HlysCBSRe | TCCTGCTTGTTATCAGCACA | 706 278–706 298 |  |  |
BHWA1_RS02885: hemolysin activation protein | ||||
Hlys3-1Fo | CTATTGGAGAGCGTACATCT | 503 577–503 597 | 1014 | 58 |
Hlys3-1Re | TACCCTGTACCTACAGAACA | 504 571–504 591 |  |  |
BHWA1_RS09085: hemolysin III channel protein | ||||
Hlys3-2Fo | CTCCTCCCGTTCAATATGTA | 2 156 200–2 156 220 | 974 | 58 |
Hlys3-2Re | AATCCGCCATGTAAAACTGC | 2 157 154–2 157 174 |  |  |