Figure 4

Heat map depicting the expression of immune genes of juvenile Crassostrea gigas infected with OsHV-1, Vibrio aestuarianus and V. tasmaniensis LGP32. The expression of each target gene was normalised to the transcript level of control groups (juvenile injected with sterile seawater) at each sampling time point (1, 2, 3 and 4 days post-injection). Hierarchical clustering confirms that juvenile have a distinct anti-viral and anti-bacterial response. Juveniles in the early stages of OsHV-1 infection (J1) cluster separately to juveniles in the early (J3), mid (J2) and late (J4) stages of Vibrio infection. No distinction in the transcriptional response was observed between juvenile infected with V. aestuarianus or V.LGP32. Juveniles in the early stages of OsHV-1 infection have a transcriptional response involving the up-regulation of genes in cluster G2, whereas Vibrio infection results in the up-regulation of genes in cluster G1.