Table 1 Primers for qRT-PCR
Gene | Nucleotide sequence (5′–3′) | Tm (°C) | Length (bp) |
|---|---|---|---|
β-actin | Forward TGCGGGACATCAAGGAGAAG | 57.4 | 216 |
Reverse AGTTGAAGGTGGTCTCGTGG | 57.4 | ||
NLRP3 | Forward GGAGGAGGAGGAAGAGGAGATA | 59.5 | 147 |
Reverse AGGACTGAGAAGATGCCACTAC | 57.7 | ||
ASC | Forward ACAACAAACCAGCACTGCAC | 55.4 | 126 |
Reverse CTGCCTGGTACTGCTCTTCC | 59.5 | ||
Caspase-1 | Forward GAAGGAGAAGAGGAGGCTGTT | 57.6 | 268 |
Reverse AGATTGTGAACCTGTGGAGAGT | 55.8 | ||
IL-1β | Forward TCTGCATGAGCTTTGTGCAAG | 55.6 | 225 |
Reverse ACAGGGCAGACTCGAATTCAAC | 57.7 | ||
IL-18 | Forward AGTAACCATCTCTGTGCAGTGT | 55.8 | 155 |
Reverse TCTTATCATCATGTCCAGGAAC | 53.9 | ||
TNF-α | Forward CGCTCTTCTGCCTACTGCACTTC | 61.3 | 164 |
Reverse CTGTCCCTCGGCTTTGACATT | 57.6 | ||
IL-6 | Forward CCAGGAACCCAGCTATGAAC | 57.4 | 142 |
Reverse CTGCACAGCCTCGACATT | 54.9 | ||
IL-8 | Forward CAGAGCCAGGAAGAGACT | 54.9 | 461 |
Reverse GACCAGCACAGGAATGAG | 54.9 | ||
IL-10 | Forward GCATCCACTTCCAGGCCA | 57.2 | 176 |
Reverse CTTCCTCATCTTCATCGTCA | 53.4 | ||
COX-2 | Forward CTGTCCCATCCCTCGGTTTA | 54.4 | 105 |
Reverse TCTCTGAGCACTGTCCGTAAT | 54.4 |