From: Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse
Primers | Oligonucleotide sequence (5′ to 3′) | Target genes | Products (bp) |
---|---|---|---|
Cpyk-F | GGGGTACCTTGTCCAATATAAAGCGATGAC KpnI, underlined | The pyk containing the promoter region | 1934 |
Cpyk-R | CGGGATCCAATGCCGGATTTTCCGTCAGCG BamHI, underlined | ||
3 × Flag-R | GCTCTAGACAGGGATGCCACCCGGGATC XbaI, underlined | The 3 × Flag tag of p3 × Flag-CMV-14 | |
pyk-UF | CGGGATCCCGGGGGTTATGGAAAGCAACT | The upstream fragment of pyk | 947 |
pyk-UR | TGACGACGCAATGCAGGCTCGAGGGTGAAGGTCTGG | ||
pyk -DF | CCAGACCTTCACCCTCGAGCCTGCATTGCGTCGTCA | The downstream fragment of pyk | 995 |
pyk- DR | CGGGATCCCGTACGGGTGCGGGTGTTTC | ||
In-pyk-F | ATGCCGTGCTGAAGGAAGAG | The inside fragment of pyk gene | 493 |
In-pyk-R | GCGTCAATGATGGTCGAATAGG | ||
Out-pyk-F | GGGGTACCCCGACGGTGGGAAGGCAAAG | The outside fragment of pyk gene | 1885 |
Out-pyk-R | CGGGATCCCGGAGCGGCTCCAGAAATCG | ||
RT-pyk-F | AAAACTGCATCTGGTGGCTG | pyk | 207 |
RT-pyk-R | GGGCGCTGGATAAAGGAAAG | ||
RT-upstream-F | ATGACATCAATCGCACGCTG | bab_RS24315 | 161 |
RT-upstream-R | GAAATTCTTTTGGGCGTCGC | ||
RT-downstream-F | AAGCTGCAAAACCCTGATCG | bab_RS24325 | 209 |
RT-downstream-R | AGCTTGATTGTTCCCCGGTA | ||
RT-GAPDH-F | GACATTCAGGTCGTCGCCATCA | gapdh | 188 |
RT-GAPDH-R | TCTTCCTTCCACGGCAGTTCGG |