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Table 2 Primers used in the study

From: Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse

Primers

Oligonucleotide sequence (5′ to 3′)

Target genes

Products (bp)

Cpyk-F

GGGGTACCTTGTCCAATATAAAGCGATGAC KpnI, underlined

The pyk containing the promoter region

1934

Cpyk-R

CGGGATCCAATGCCGGATTTTCCGTCAGCG BamHI, underlined

3 × Flag-R

GCTCTAGACAGGGATGCCACCCGGGATC XbaI, underlined

The 3 × Flag tag of p3 × Flag-CMV-14

 

pyk-UF

CGGGATCCCGGGGGTTATGGAAAGCAACT

The upstream fragment of pyk

947

pyk-UR

TGACGACGCAATGCAGGCTCGAGGGTGAAGGTCTGG

pyk -DF

CCAGACCTTCACCCTCGAGCCTGCATTGCGTCGTCA

The downstream fragment of pyk

995

pyk- DR

CGGGATCCCGTACGGGTGCGGGTGTTTC

In-pyk-F

ATGCCGTGCTGAAGGAAGAG

The inside fragment of pyk gene

493

In-pyk-R

GCGTCAATGATGGTCGAATAGG

Out-pyk-F

GGGGTACCCCGACGGTGGGAAGGCAAAG

The outside fragment of pyk gene

1885

Out-pyk-R

CGGGATCCCGGAGCGGCTCCAGAAATCG

RT-pyk-F

AAAACTGCATCTGGTGGCTG

pyk

207

RT-pyk-R

GGGCGCTGGATAAAGGAAAG

RT-upstream-F

ATGACATCAATCGCACGCTG

bab_RS24315

161

RT-upstream-R

GAAATTCTTTTGGGCGTCGC

RT-downstream-F

AAGCTGCAAAACCCTGATCG

bab_RS24325

209

RT-downstream-R

AGCTTGATTGTTCCCCGGTA

RT-GAPDH-F

GACATTCAGGTCGTCGCCATCA

gapdh

188

RT-GAPDH-R

TCTTCCTTCCACGGCAGTTCGG