Figure 3
From: Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells
siRNA against chGRP78 in DF1 cells. The cells were transfected with siRNA (50Â pmol) against chGRP78 for 6Â h, and the cells were then infected with ALV-J at an MOI of 1 for 72Â h. A The siRNA effects on chGRP78 were detected with real-time PCR. B The infection/replication of ALV-J was analysed with Western blot. Lanes 1, 2, 3 and 4, DF1 cells transfected with siRNA 519, 611, 366 and Mix (co-transfected with 519, 611 and 366), respectively, against chGRP78. Lane 5, DF1 cells transfected with control siRNA. C TCID50 analysis of the viral titres.