Figure 4From: The activation of p38MAPK and JNK pathways in bovine herpesvirus 1 infected MDBK cells The effect of ROS scavenger NAC on BHV-1 stimulated MAPK pathways. MDBK cells were first mock pretreated with DMSO control or pretreated with NAC at the indicated concentrations for 1 h, then mock infected or infected with BHV-1 at an MOI of 10 along with NAC or DMSO control. At 24 hpi, cell lysates were prepared and phosphorylated forms of JNK, c-Jun, p38MAPK and Erk1/2 were detected using Western blotting. Data represent three independent experiments.Back to article page