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Table 1 List of mutants screened in the initial round of screening in an intramammary sheep infection model

From: Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep

Group I-15 mutants

Group II-17 mutants

Group III-19 mutants

Mutant

Genes disrupted

Mutant

Genes disrupted

Mutant

Genes disrupted

Apo7

Hypothetical Protein (MAG1890)

Apo2-12

Hypothetical protein, predicted lipoprotein (MAG1050)

3-20

NADH oxidase (nox)

Apo6

Sugar isomerase (araD)

1-3-0

Hypothetical protein (MAG2810)

13

VpmaX

26

Oligopeptide transporter ATP binding protein (oppF)

15-2

Hypothetical protein, predicted lipoprotein (MAG6200)

14

DNA methylase (MAG1790)

3-15

Elongation factor G (fusA)

170

Hypothetical protein (MAG0110)

137

Hypothetical protein (MAG4460)

6-9-2

Oligopeptide ABC transporter permease (oppC)

3-29

Hypothetical protein/Vpma like predicted lipoprotein (MAG2540)

8-1

Deoxyguaosine kinase (dgk)

6-14

Hexose P transport protein (uhpT)

4-1-0

GTP-dependent nucleic acid-binding protein (engD)

1-2-0

Pentitol posphotransferase

enzyme II (MAG6360)

6-21

Cell division protein (mraZ)

8 + 1-4

ABC transporter ATP-binding protein (MAG5960)

96-2

ATP synthase (atpA)

6-29

Pyruvate dehydrogenase beta subunit (pdhB)

8 + 1-8

Transport protein SGAT (ulaA)

5-1

Hypothetical protein (MAG0250)

6-32

Phosphate acetyl transferase (eutD)

3-4-0

Hypothetical protein (MAG3390)

6-13

Hypothetical protein (MAG1570)

7 + 1-8

Hypothetical protein (MAG4010)

9-28

Conserved hypothetical protein (MAG0390)

7 + 1-3

Conserved hypothetical protein (MAG3650)

9-40

Glycerol ABC transporter permease component (gtsB)

62

Lipoate Protein Ligase A (lplA)

23

Aminopeptidase (MAG5520)

3-1

Phosphoenol-

pyruvate phosphotransferase ( ptsL )

3-1

Phosphoenolpyruvate Phosphotransferase ( ptsL )

3-1

Phosphoenolpyruvate Phosphotransferase ( ptsL )

8 + 1-5

Ribonuclease H II ( rnhB )

8 + 1-5

Ribonuclease H II ( rnhB )

  

9-31

Alcohol dehydrogenase (adhT) and NADH oxidase (nox)

  

9-31

Alcohol dehydrogenase (adhT) and NADH oxidase (nox)

6-15

Esterase/lipase ( lip )

  

6-15

Esterase/lipase ( lip )

  

81-1

Oligopeptide ABC transporter permease (oppB)

81-1

Oligopeptide ABC transporter permease (oppB)

  

9-8

Dihydrolipoamide dehydrogenase (pdhD)

6-12

Transcriptional regulator (MAG6310)

  

212

Type III restriction modification system (MAG1530)

San5

Predicted lipoprotein (MAG3120)

  

9-16

Conserved hypothetical protein (MAG1450)

3-23

Lipoprotein (MAG6410)

    

6-15

VpmaY

  1. Mutants kept common (“watermarks”) between the groups are underlined and those shortlisted for secondary screening showing >95% absence are in italics.