Figure 3
Cytokine responses in PBMC, BAL and TBLN. Pigs were inoculated with 1 × 104 EID50 (low, circles) or 1 × 107 EID50 (high, squares) of A/Sw/Eng/1353/09 virus by AERO (open symbols) or IN (solid symbols) or by contact with the high dose AERO infected pigs (open diamonds) or by contact with the high dose IN infected pigs (solid diamonds) as indicated on the figure. A Numbers of IFNγ secreting cells in PBMC, BAL and TBLN at 14 dpi or 16 dpc were determined by ELISPOT. B The percentage of IFNγ producing cells in BAL was determined by intracellular cytokine staining and flow cytometry at 14 dpi or 16 dpc. Cytokine expression is presented as a percentage of the total number of cells in CD4+, CD4+CD8+ and CD8+ cells. Each data point represents an individual within the indicated group and the horizontal lines indicates the mean. C Box and whisker plots of the scores of PC1 (29.59% explained variance) for immune response in each treatment group. Each boxplot represents the distribution of estimates for pigs in the indicated groups.