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Figure 6 | Veterinary Research

Figure 6

From: L-BSE experimentally transmitted to sheep presents as a unique disease phenotype

Figure 6

WB of brain and LRS from 48/13 (the VRQ/VRQ recipient with LRS involvement). A Detection with mAb Sha31; B detection with mAb P4. (M, molecular markers; B+, bovine C-BSE control; S+, ovine classical scrapie control; Lane 1, ovine C-BSE brain; Lane 2, ovine C-BSE lymphoid tissue; Lane 3, ovine L-BSE lymphoid tissue (case 48/13); Lane 4, ovine L-BSE brain (case 48/13). 1 min exposure). The lymphoid tissue from the ovine C-BSE sample (lane 2) and L-BSE recipient (lane 3) show similar but not identical molecular profiles to each other with an overall higher mass migration pattern. However only the L-BSE sample is detected by mAb P4. The ovine C-BSE brain sample exhibits the expected profile characteristics of a low molecular mass migration, predominant diglycosylated band and mimimal detection with mAb P4. The ovine L-BSE brain sample (lane 4) exhibits a molecular mass migration that is higher than expected when compared to the primary and sub passaged results (Figures 4 and 5), appearing similar to the ovine scrapie control. However, the equal intensity of di and monoglycosylated bands can be observed. The sample was also detected by mAb P4.

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