Skip to main content
Figure 6 | Veterinary Research

Figure 6

From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

Figure 6

Pro-inflammatory interleukins genes transcription in cultured canine endometrial epithelial and stromal cells after Escherichia coli or LPS cell stimulation. Relative mRNA expression (qRT-PCR) of IL- (A), IL- (B), IL-6 (C), TNFα (D) in cultured canine endometrial epithelial and stromal cells in response to hemolytic E. coli (Pyo18), non-hemolytic E. coli (Pyo14) and the isogenic mutant of Pyo18 (Pyo18ΔhlyA). Treatment with 1 μg/mL of LPS was used as a positive control. Data are mean ± SEM (n = 3 uteri with two replicates for all stimuli). Transcription levels (fold increase) are normalized with those of non-stimulated cells a vs b p < 0.05 [(Epithelia cells: IL-, IL-6; stromal cells: IL-, IL-, TNFα]; a vs b p < 0.01 (stromal cells: IL-6); a vs c p < 0.05 (stromal cells: TNFα); * vs **p < 0.05 (IL-) * vs **p < 0.01 (IL-); p < 0.05 (1 vs 4 h: IL-, stromal cells); p < 0.01 (1 vs 4 h: IL-6, epithelia cells).

Back to article page