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Table 1 Primer sequences used for RT-PCR

From: Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

Gene

Accession number

Sequence (5′–3′)

Annealing temperature (°C)

Amplicon size (bp)

MyD88

XM_534223

FW-ACTATCGGCTGAAGTTGTGTGTGT

RV-TGGTGTAGTCACAGACAGTGATGAA

58.3

276

TRAM

NM_001204337

FW-GGGTGTCAGGAAGTCGAAAATA

RV-CTGCGTGCAGTATCACAAACTT

56.8

266

TRAF6

XM_003432322

FW-AAACTGTGAAAACAGCTGTGGA

RV-CAGTTCATGCAAGAAACCTGTC

56.1

564

TRIF

XM_849573

FW-CTTCCAAAGCCCATAGAGGA

RV-AAGCGGTGTCTTCTACAGGAA

55.4

487

IRF3

XM_005616307

FW-GGTGCCTACACTCCTGGAAA

RV-CTTCATCAGGCACCAAGAGC

56.0

418

NFkB

NM_001003344

FW-TGTTTCACTTGGATCCTTTGAC

RV-AGATCCCATCCTCACAGTGTTT

55.4

327

IL-

NM_001037971

FW-CACCAGTGAAATGATGGCTTAC

RV-CTCATGTGGAACACCACTTGTT

56.0

453

IL-6

NM_001003301

FW-GTACATCCTCGGCAAAATCTCT

RV-GGATGAGGTGAATTGTTGTGTG

56.0

410

IL-8

NM_001003200

FW-TTGGCAGCTTTTGTCCTT

RV-GGGCCACTGTCAATCACT

52.5

149

IL-10

NM_001003077

FW-AAGCTGGACAACATACTGCTGA

RV-TGTCAAACTCACTCATGGCTTT

56

310

TNF-α

NM_001003244

FW-TGACAAGCCAGTAGCTCATGTT

RV-CGGCAAAGTCCAGATAGTTAGG

56

410

IL-2

NM_001003305

FW-TTGTCGCAAACAGTGCACCTA

RV-CCTGGAGAGTTGGGGGTTCT

60

131

IL-4

NM_001003159

FW- CTCACCAGCACCTTTGTCCA

RV- GTCAGCTCCATGCACGAGTC

60

107

IFN-β

NM_001135787

FW- CAGTAGATGCATCCTCCAAACA

RV- GACTATTGTCCAGGCACAGATG

55.9

493

IFN-γ

NM_001003174

FW-GCTGTAACTGTCAGGCCATGTTT

RV-TGTTTTGTCACTCTCCTCTCTCCA

60

140