Figure 1

Cell cycle distribution of PCV2-infected PK15 cells. A Flow cytometry analysis of cell cycle subversion in mock-infected or PCV2-infected PK15 asynchronously growing cells. Cells were fixed in ethanol at 12, 24, and 48 h post-infection (pi) with 0.5 and 1 MOI of PCV2, DNA was stained with propidium iodide and applied to flow cytometry. B Flow cytometric analysis of cell cycle profiles in serum-starved PK15 cells with mock or 1 MOI of PCV2 infection. Cells were fixed in ethanol at the indicated time and stained with PI. The percentages of each cell cycle phase (G0/G1, G2, S) were output and analyzed by ModFit LT software in the right panel. C qPCR determined the level of PCV2 ORF1 genomic DNA at the indicated infection time in PCV2-infected asynchronic and G0/G1 synchronic cells. D Cell cycle profiles were measured after 1 MOI PCV2 or UV-inactivated PCV2 infected cells for 24 h. The data are mean ± SEM of three independent experiments. *p < 0.05, versus mock infected cells at the same time points (A, B, D). ^# p < 0.05, ^## p < 0.01 versus 6 h pi, in the same infected cells (C).