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Figure 2 | Veterinary Research

Figure 2

From: Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae

Figure 2

APP-CCE specific IL-17A-producing CD4 + T cells in lung, blood, tracheobronchial lymph nodes and tonsils. Cells isolated from lung, blood (PBMC), tracheobronchial lymph nodes (TBLN) and tonsils were incubated overnight with APP crude capsular extract (APP-CCE), medium or PMA/Ionomycin. Living lymphocytes were gated (not shown; see Additional file 1) and further analyzed for the expression of IL-17A and CD4. A For the lung, data from representative animals from different groups are displayed: #5 and #8 for the acute phase, designated as “high responder” and “low responder” respectively; #12 and #16 for the chronic phase designated as “high responder” and “low responder” respectively; #6, designated as non-responder and control #23. Approximately 1 × 106 (APP and medium) and 2 × 105 (PMA/Ionomycin) cells are shown in the contour plots. Numbers displayed within the contour plots indicate the percentages of IL-17A+ CD4+ T cells within total CD4+ T cells. B Frequency of IL-17A+ CD4+ T cells within total CD4+ T cells in lung, blood (PBMC), tracheobronchial lymph node (TBLN) and tonsils of all infected animals (red dots) and control animals (blue dots) during acute and chronic phase. Numbers next to colored dots indicate numbers of individual animals. Median percent values are indicated by black bars. Medium-corrected percent values are presented (% of IL-17A+ CD4+ T cells within total CD4+ T cells for APP-CCE stimulation minus % of IL-17A+ CD4+ T cells within total CD4+ T cells for medium incubation).

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