Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture

Figure 4

Susceptibility of different strains of carp to an infection with CEV from the koi, genogroup IIa (Experiment no. Co IV). Depicted is carp edema virus load (A) and replication (B) in the gills of koi (Koi) and common carp from the strains Amur wild carp (AS), Ropsha (Rop) and Prerov (PS) during cohabitation with koi affected by KSD. CEV load was measured by qPCR as copy numbers of virus specific DNA. As a surrogate for CEV replication, the expression of mRNA encoding the gene of the CEV core protein P4a (CEV P4a) was determined. The data on virus load and expression of viral mRNA are shown as box plots indicating the range of 25–75% of the values in the box (±minimum and maximum values) of genome copies in 250 ng of isolated DNA from n = 4 fish (virus load) or of the copy number of mRNA encoding the viral P4a gene normalised against 100 000 copies of the carp 40S ribosomal protein S11 (CEV replication). Symbols “+” and “□” indicate mean and median respectively. Different letters indicate significant differences at p ≤ 0.05 between carp strains.

Back to article page

Veterinary Research

ISSN: 1297-9716