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Figure 1 | Veterinary Research

Figure 1

From: Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate

Figure 1

Characterization of mutant strain ∆22915. A Schematic of BAB_RS22915 gene deletion. A 1036-bp fragment was deleted from the BAB_RS22915 coding sequence. B Transcription of BAB_RS22915 and flanking genes in ∆22915. Transcription of BAB_RS22915 was abolished and had no influence on transcriptional BAB_RS22910 and BAB_RS22920. Data were presented as mean ± SD, and analyzed using a Student’s t test. C Silver staining of bacterial LPS. No difference was seen in LPS patterns between ∆22915 and wild-type strain S2308. Lane Marker: Prestain page ruler (Thermo Scientific, USA); Lane S2308: B. abortus S2308 LPS; Lane ∆22915: ∆22915 LPS. D Bacterial growth curves. Strains ∆22915 and S2308 were cultured in TSB media, and OD600 was measured every 4 h to monitor growth. Data were presented as mean ± SD, and analyzed with two-way ANOVA. **P < 0.01; ***P < 0.001.

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