Enhancing the toolbox to study IL-17A in cattle and sheep

Table 2 Isotype control mabs and control pab used in the evaluation of the commercial anti-IL-17A antibodies

Control antibody used in the intracellular staining (CHO cells)	Clone	Source	Immunogen/host raised in	Isotype	Antibody conjugation, if present
a	N/A	Professor Waithaka Mwangi, A & M University, Texas, USA	Bovine CD34 construct/rabbit	Rabbit IgG	None
b, c and d	VPM 21	Moredun	Border disease virus/mouse	Mouse IgG1	None
d	VPM22	Moredun	Border disease virus/mouse	Mouse IgG2b	None

The two mabs VPM21 and VPM22 (both MRI, Edinburgh, UK) and control pab (raised against bovine CD34; A & M University, Texas, USA) were derived from non-commercial sources. The hybridoma cell lines were grown in-house to generate mabs that were then adjusted for concentration to match that of the anti-IL-17A mabs listed in Table 1. The antibodies were assigned a code letter in lower case that corresponds with the commercial antibodies listed in Table 1 (denoted by the equivalent uppercase letter) for the intracellular staining protocol followed in “Evaluation of commercial antibodies section”.

ISSN: 1297-9716