Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Ahlberg, Viktor; Hjertner, Bernt; Wallgren, Per; Hellman, Stina; Lövgren Bengtsson, Karin; Fossum, Caroline

doi:10.1186/s13567-017-0437-2

Veterinary Research

Table 1 Primer details and qPCR conditions

From: Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Gene	Primer sequence	Anneal temp (°C)	Primer conc (nM)	Eff (%)^a	r ²	Melt point (°C)	References
CXCL8	F: AGCCAGGAAGAGACTAGAAAGAAA R: TTGGGGTGGAAAGGTGTG	56	500	97	0.998	81.5	New design
GAPDH^b	F: ACACTCACTCTTCTACCTTTG R: CAAATTCATTGTCGTACCAG	56	500	94	0.998	80	[54]
HPRT^b	F: GGTCAAGCAGCATAATCCAAAG R: CAAGGGCATAGCCTACCACAA	60	500	100	0.996	80	[55]
IFITM3	F: ATCAACATCCGAAGCGAGACC R: GGAAAATTACCAGGGAGCCAGTG	56	500	96	0.999	85.5	New design
IFN-α	F: AGCCTCCTGCACCAGTTCTG R: TCACAGCCAGGATGGAGTCC	60	500	100	0.997	84.5	[20]
IFN-β	F: TAGCACTGGCTGGAATGAAACC R: TCAGGTGAAGAATGGTCATGTCT	58	400	104	0.993	79.5	[20]
IFN-γ	F: TGGTAGCTCTGGGAAACTGAATG R: GGCTTTGCGCTGGATCTG	60	400	102	0.998	76.5	[31]
IL1B	F: GTGATGGCTAACTACGGTGACAA R: CTCCCATTTCTCAGAGAACCAAG	60	400	91	0.999	79.5	[56]
IL6	F: CTGGCAGAAAACAACCTGAACC R: TGATTCTCATCAAGCAGGTCTCC	60	400	98	0.994	77.5	[57]
IL10	F: CGGCGCTGTCATCAATTTCTG R: CCCCTCTCTTGGAGCTTGCTA	60	400	100	0.995	80.5	[57]
IL12B	F: TCTTGGGAGGGTCTGGTTTG R: AAGCTGTTCACAAGCTCAAGTATGA	61	400	96	0.999	76.5	[31]
IL17A	F: CAGACGGCCCTCAGATTACTCCA R: AGCCCACTGTCACCATCACTTTCT	61	400	91	0.993	84.5	New design
PPIA^b	F: GCAGACAAAGTTCCAAAGACAG R: AGATGCCAGGACCCGTATG	60	400	92	0.999	80.5	[58]
RPL32^b	F: CGGAAGTTTCTGGTACACAATGTAA R: TGGAAGAGACGTTGTGAGCAA	55	300	97	0.997	77	[20]
SPP1	F: TTGGACAGCCAAGAGAAGGACAGT R: GCTCATTGCTCCCATCATAGGTCTTG	56	300	93	0.997	82.5	[20]
STING	F: TTACATCGGGTACCTGCGGC R: CCGAGTACGTTCTTGTGGCG	56	500	101	0.992	82	[20]
TGFB1	F: TACGCCAAGGAGGTCACCC R: CAGCTCTGCCCGAGAGAGC	60	400	90	0.992	83	[59]
TLR2	F: GGCAAGTGGATTATTGACAACATC R: ACCACTCGCTCTTCACAAAGTTC	60	500	94	0.997	78.5	[60]
TLR4	F: CTTCACTACAGAGACTTCATTC R: ACACCACGACAATAACCT	54	500	89	0.999	79	[61]
TNFA	F: AGCCTCTTCTCCTTCCTCCTG R: GAGACGATGATCTGAGTCCTTGG	60	400	91	0.993	84	[31]
YWHAZ^b	F: ATTGGGTCTGGCCCTTAACT R: GCGTGCTGTCTTTGTATGACTC	58	400	101	0.997	78.5	[20]

Conditions optimized or re-optimized for reagents and equipment described in “Materials and methods”.
^aPCR efficiency estimated on serial dilutions of reference cDNA.
^bReference gene used for normalization.

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ISSN: 1297-9716

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