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Table 2 Origin of samples, RT-qPCR results and threshold cycle values

From: Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis

Sample source

Number of samples analysed by RT-qPCR

RT-qPCR positive result (%)

Threshold cycle for positive samples—median; range (relative copy number—median; range)

Cats with FIP

 Tissue

260

235 (90.4)

26.2; 12.4 to 39.7 (1.1 × 104; 1.2 × 108 to 1.2)

 Fluid

51

40 (78.4)

31.2; 19.4 to 38.5 (373; 1.1 × 106 to 2.7)

  Body cavity fluid

35

32 (91.4)

30.9; 19.4 to 38.5 (457; 1.2 × 108 to 1.2)

   Abdominal

23

21 (91.3)

30.8; 22.2 to 38.5 (488; 1.6 × 105 to 2.7)

   Pleural

9

9 (100.0)

29.6; 19.4 to 35.0 (1.1 × 10−3 1.2 × 108 to 29)

   Pericardial

2

1 (50.0)

38.5 (2.7)

   Unrecorded

1

1 (100.0)

37.0 (7.5)

  CSF

14a

7 (50.0)

34.8; 27.9 to 36.5 (33; 3.4 × 103 to 11)

  Aqueous humour

2

1 (50.0)

33.7 (6.9)

 Faeces

48

31 (64.6)

30.9; 15.8 to 39.7 (457; 1.2 × 107 to 1.2)

  Whole faeces

42

28 (66.7)

31.0; 15.8 to 37.7 (427; 1.2 × 107 to 4.7)

  Faecal swab

6

3 (50.0)

30.5; 30.5 to 39.7 (598; 598 to 1.2)

Cats without FIP

 Tissue

258

20 (7.8)

36.2; 26.4 to 38.5 (12.9; 9.4 × 103 to 2.7)

 Fluid

47

1 (2.1)

36.4 (11)

  Body cavity

28

1 (3.6)

36.4 (11)

   Abdominal

13

1 (7.8)

36.4 (11)

   Pleural

12

0

 

   Pericardial

3

0

 

  CSF

19

0

 

 Faeces

35

7 (20.0)

 

  Whole faeces

33

7 (20.0)

33.6; 25.7 to 38.6 (74; 1.5 × 104 to 2.6)

  Faecal swab

2

0

33.6; 25.7 to 38.6 (74; 1.5 × 104 to 2.6)

  1. aAll but one sample was obtained from the cerebellomedullary cistern, one sample was collected from the lumbar subarachnoid space.