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Table 1 Bacterial strains and plasmids utilized in this study

From: Salmonella Typhimurium, the major causative agent of foodborne illness inactivated by a phage lysis system provides effective protection against lethal challenge by induction of robust cell-mediated immune responses and activation of dendritic cells

Strain/plasmids

Description

References

E. coli

 JOL232 (χ6212)

F-λ-φ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4

Lab stock

Salmonella Typhimurium

 JOL 401

Wild type isolate from porcine, challenge strain

Lab stock

 JOL1311

Δasd, used as base vaccine strain

Lab stock

 JOL1950

JOL1311 containing pJHL464

This study

Plasmids

 pJHL172

asd + vector, pBR ori plasmid harboring cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E

[22]

 pJHL319

T-easy vector harboring E gene ghost cassette with the convergent promoter system

This study

 pJHL360

T-easy vector harboring the R ghost cassette with the convergent promoter system

This study

 pJHL464

asd+ vector, pBR ori plasmid harboring cI857/λPR promoter, araC P araBAD , the R ghost cassette composed of S, R and R1/Rz genes

This study