Figure 6

Progression of total lung lesion burden and Type heterogeneity following the course of M. bovis infection. A Morphometric analysis of the total lesion area performed on H&E-stained sections of the global lung (5 lobes) of mice following intranasal infection with M. bovis. Histograms represent the mean percentage of lesion area within the total lung ± s.e.m at 1 to 9 weeks post-infection (n = 5 to 8 mice per time point). B High resolution scanned slides of H&E stained lung from representative C3HeB/FeJ mice at different weeks post-infection (W3 to W9) showing the characteristic evolution of Type I (top images), Type II (middle images) and Type III (down images) lesions over the course of infection. C Histometric analysis of the relative percentage of each Type of lesion within the total lesion area performed on H&E-stained sections of the global lung (5 lobes) of mice following intranasal infection with M. bovis. Histograms represent the mean percentage of each lesion Type over the total percentage (100%) of lesion area ± s.e.m at 1 to 9 weeks post-infection (n = 5 to 8 mice per time point). The dotted rectangle indicate the presence, at a low rate (0.7%), of emerging Type I lesions as soon as 5 weeks post-infection. The dotted red line delimitates the critical early phase of infection (week 5).