Table 1 Primer sequences for qRT-PCR analyses of cytokine transcripts
Gene | Forward primer | Probe | Reverse primer | Accession no. |
|---|---|---|---|---|
Chicken | ||||
 28S | GGCGAAGCCAGAGGAAACT | AGGACCGCTACGGACCTCCACCA | GACGACCGATTTGCACGTC | AH001604 |
 IL-1β | GCTCTACATGTCGTGTGTGATGAG | CCACACTGCAGCTGGAGGAAGCC | TGTCGATGTCCCGCATGA | NM_204524 |
 IL-6 | GCTCGCCGGCTTCGA | AGGAGAAATGCCTGACGAAGCTCTCCA | GGTAGGTCTGAAAGGCGAACAG | NM_204628 |
 CXCLi1 | TGGCTCTTCTCCTGATCTCAATG | TCGCTGAACGTGCTTGAGCCATACCTT | GCACTGGCATCGGAGTTCA | Y14971 |
IL-10 | CATGCTGCTGGGCCTGAA | CGACGATTCGGCGCTGTCACC | CGTCTCCTTGATCTGCTTGATG | NM_001004414 |
Human | ||||
 28S | GAATTCATGGACGACACGAG |  | ACTGTGACAGACCATTCCCA | NR_003279 |
 IL-6 | CCAGAGCTGTGCAGATGAGT |  | CTGCGCAGAATGAGATGAGT | NM_000600 |
 IL-10 | GAGGCTACGGCGCTGTCAT |  | TGCTCCACGGCCTTGCT | NM_000572 |
Mouse | ||||
 β-actin | TCCAGCCTTCCTTCTTGGGT |  | GCACTGTGTTGGCATAGAGGT | NM_007393 |
 IL-6 | GGGACTGATGCTGGTGACAACC |  | GGAGAGCATTGGAAATTGGG | NM_031168 |
 IL-10 | CGATGACGGGCCAGTGAGAATG |  | TCAACACGTGGGCAGGATAGGCT | NM_010548 |