Figure 5
From: Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection
Prediction analysis of miRNA-target interaction, results of luciferase assay, and gene expression of immunoproteasome components. A Prediction of target sequence in miR-125b/PSMB8 and miR-125b/PSMB9 binding. The highest score obtained from RNAhybrid prediction (−15 kcal/mol, scoring criteria) was used for the design of the luciferase reporter assay. B Firefly luciferase activity was measured and normalized by the Renilla luciferase activity. C Gene expression of PSMB8 and PSMB9 in MLN samples at 2 dpi. Results are represented as mean ratio ± SEM from four independent transfection experiments. **P < 0.01 and *P < 0.05.