Figure 3From: Effects of temperature on Veronaea botryosa infections in white sturgeon Acipenser transmontanus and fungal induced cytotoxicity of fish cell linesHistologic findings in white sturgeon challenged by intramuscular injection with Veronaea botryosa at 18 °C. A Liver, with large pale staining area of coagulative necrosis (N). H&E stain, Bar = 200 μm. B Severe splenomegaly, white pulp areas are diffusely accentuated by extensive pale staining sinusoidal edema and congestion. H&E stain, Bar = 200 μm. C Liver, with vascular occlusion (V), associated necrotic debris, perivasculitis and hepatocellular necrosis secondary to angioinvasion by the fungus. H&E stain, Bar = 100 μm. D PAS stained section of liver, corresponding to image (C), reveals large numbers of magenta hyphae extending from the vascular lumen into hepatic sinusoids. Bar = 100 μm. E Heart, with fungal hyphae, macrophages and lymphocytes in the lumen of an epicardial arteriole (arrows). H&E stain, Bar = 20 μm. F Gill, with a collection of giant cells and pigmented hyphae (arrow) at the base of a lamellar trough. H&E stain, Bar = 20 μm.Back to article page