Figure 5

Validation of NFAT5 and NPEPPS as direct targets of ssc-miR-122. A mRNA expression of NFAT5 and NPEPPS in lung tissues from uninfected or infected LW and YL pigs. B mRNA expression of NFAT5 and NPEPPS in PK15 cells transfected with the ssc-miR-122 mimic. C Protein expression of NFAT5 and NPEPPS in PK15 cells transfected with ssc-miR-122 mimic. D Diagram of the predicted miR-122 targeting site within the 3′ UTR of NFAT5 and NPEPPS (3′ UTR WT). The mutated 3′ UTR (3′ UTR MT) contains a mutated sequence which is not complementary to the ssc-miR-122 seed sequence (GUGA).E Relative fluorescence activity of the luciferase constructs harboring the wildtype or mutant 3′ UTR of either NFAT5 or NPEPPS genes in PK15 cells with or without the ssc-miR-122 mimic. SE from the mean are labeled on the bar using vertical lines. *, ** and *** indicate significance at the P value threshold levels of 0.05, 0.01 and 0.001, respectively.