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Figure 2 | Veterinary Research

Figure 2

From: Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids

Figure 2

Temporal pattern of Stx neutralizing titers in calves’ sera. Antibody titers were determined before (ac = ante colostrum) and after (pc = post-colostral) colostrum intake as well as in the 3rd, 11th, 16th, 26th and 55th week of life. Animals were grouped according to their vaccination status (VAC+ = rStxMUT-vaccinated; VAC− = placebo control) and the supplementation of vitamin E (VitEH = high supplementation; VitEM = moderate supplementation). Arrows indicate active vaccination in the 5th and 8th week of life. A Temporal pattern of Stx1 neutralizing titers. A titer of 450 was attributed to those samples with a relative cell activity < 30% (detection limit, dashed line). B Temporal pattern of Stx2 neutralizing titers. A titer of 300 was attributed to those samples with a relative cell activity < 20% (detection limit, dashed line). Results of Vero cell neutralization assay expressed as box plots with 5–95% interquartile ranges. Different letters indicate significant differences between the groups at each time point separately based on Dunn’s test after a Kruskal–Wallis test.

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