Figure 2From: Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoidsTemporal pattern of Stx neutralizing titers in calves’ sera. Antibody titers were determined before (ac = ante colostrum) and after (pc = post-colostral) colostrum intake as well as in the 3rd, 11th, 16th, 26th and 55th week of life. Animals were grouped according to their vaccination status (VAC+ = rStxMUT-vaccinated; VAC− = placebo control) and the supplementation of vitamin E (VitEH = high supplementation; VitEM = moderate supplementation). Arrows indicate active vaccination in the 5th and 8th week of life. A Temporal pattern of Stx1 neutralizing titers. A titer of 450 was attributed to those samples with a relative cell activity < 30% (detection limit, dashed line). B Temporal pattern of Stx2 neutralizing titers. A titer of 300 was attributed to those samples with a relative cell activity < 20% (detection limit, dashed line). Results of Vero cell neutralization assay expressed as box plots with 5–95% interquartile ranges. Different letters indicate significant differences between the groups at each time point separately based on Dunn’s test after a Kruskal–Wallis test.Back to article page