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Figure 1 | Veterinary Research

Figure 1

From: Piscine orthoreovirus infection in Atlantic salmon (Salmo salar) protects against subsequent challenge with infectious hematopoietic necrosis virus (IHNV)

Figure 1

Experimental design. After acclimation and health screening (1), Atlantic salmon parr were divided in 6 × 150 L tanks and PRV cohabitation challenge was performed. Each tank contained each 40 shedders or mock-shedders (controls) and 55 cohabitants (2). After monitoring PRV infection for 4 weeks (3), IHNV bath challenge was performed (4). At this stage 32 shedders and 47 cohabitants were present in each tanks. The trial continued for 45 days after IHNV trial. (5) Sampling at selected time points continued in large tanks, while pathogenicity assessment was conducted in duplicate small 8 L bowls (10 shedders and 10 cohabitants) (tanks 1a, b and 3a, b).

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