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Figure 3 | Veterinary Research

Figure 3

From: Atrophy of primary lymphoid organs induced by Marek’s disease virus during early infection is associated with increased apoptosis, inhibition of cell proliferation and a severe B-lymphopenia

Figure 3

MDV increases apoptosis in the thymus and the bursa at 6 and 10 dpi. A Cryosections of thymus (a–f) and bursa (g–l) harvested on CTL and MDV-infected birds at 6, 10 and 14 dpi were stained by TUNEL assay (green) and the nuclei counterstained with Hoescht 33342 dye (blue). TUNEL positive cells presented green nuclei, as shown enlarged (× 3) in the corner panel (e). Apoptosis was increased in the thymus of MDV-infected chicks compared to controls at 6 dpi, especially in the medulla. For the bursa, apoptosis was increased at 6 and 10 dpi, especially in the medulla. B Quantification of the green fluorescent signal on cryosections stained by TUNEL assay. This measurement was performed by area determination with FIJI software on three pictures per organ/chick/time. The median is represented with a black line. Apoptosis was highly enhanced at 6 dpi in the thymus and the bursa and to a lesser extend at 10 dpi compared to CTL. No differences in apoptosis were observed between infected- and CTL chicks at 14 dpi

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Veterinary Research

ISSN: 1297-9716