Group | Animal id. | Foetal death (days pi)a | Placentomes/cotyledonsb | Foetal brain | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Foetus/lamb 1 | Foetus/lamb 2 | Foetus/lamb 3 | Foetus/lamb 4 | ||||||||||||||||||
HPc | DNAe | qPCRf | IFATd | HPc | DNAe | qPCRf | IFATd | HPc | DNAe | qPCRf | IFATd | HPc | DNAe | qPCRf | IFATd | HPc | DNAe | qPCRf | |||
Group 1 (105 tachyzoites, IV) | 1.1 | 38 | +++ | 6/6 | 2030.18 ± 2021.53 | 1:32 | +++ | 3/3 | 47.53 ± 22.41 | 1:256 | * | 0/3 | 0.01 | ||||||||
1.2 | 40 | +++ | 6/6 | 1990.74 ± 543.90 | 1:256 | ++ | 2/3 | 3.40 ± 3.01 | |||||||||||||
1.3 | 40 | ++ | 6/6 | 3355.17 ± 2407.07 | 1:1024 | +++ | 3/3 | 8.47 ± 2.67 | |||||||||||||
1.4 | 37 | ++ | 6/6 | 2028.50 ± 2434.11 | 1:128 | ++ | 2/3 | 14.42 ± 13.01 | 1:256 | +++ | 3/3 | 584.97 ± 403.74 | 1:32 | * | 3/3 | 97.72 ± 109.14 | β | β | β | β | |
1.5 | 35 | +++ | 6/6 | 3296.38 ± 1231.41 | 1:64 | +++ | 3/3 | 141.14 ± 69.34 | 1:32 | * | 3/3 | 56.18 ± 27.50 | |||||||||
1.6 | 38 | +++ | 6/6 | 7974.29 ± 4660.05 | 1:1024 | ++ | 2/3 | 13.26 ± 15.57 | 1:1024 | ++ | 3/3 | 16.86 ± 10.67 | |||||||||
Group 2 (104 tachyzoites, IV) | 2.1 | 32 | +++ | 6/6 | 7420.68 ± 6570.82 | 1:256 | ++ | 3/3 | 13.38 ± 6.04 | ||||||||||||
2.2 | NA | 4/4γ | 3992.48 ± 1562.24γ | 1:800 | + | 2/3 | 5.16 ± 5.59 | β | β | β | β | β | β | β | β | ||||||
2.3 | 32 | +++ | 6/6 | 4548.31 ± 4733.95 | 1:128 | + | 3/3 | 19.61 ± 12.04 | |||||||||||||
2.4 | 37 | + | 6/6 | 1930.36 ± 2034.10 | 1:256 | ++ | 2/3 | 5.54 ± 6.06 | β | β | β | β | |||||||||
2.5 | 42 | +++ | 6/6 | 6829.41 ± 3917.31 | 1:64 | + | 3/3 | 2.70 ± 0.86 | 1:64 | ++ | 3/3 | 20.29 ± 8.74 | NA | ++ | 3/3 | 11.97 ± 10.24 | 1:128 | ++ | 3/3 | 3.31 ± 2.49 | |
Group 3 (103 tachyzoites, IV) | 3.1 | 39 | ++ | 6/6 | 12 260.49 ± 9266.82 | 1:32 | ++ | 3/3 | 9. 48 ± 5.47 | 1:256 | ++ | 3/3 | 23.56 ± 13.52 | ||||||||
3.2 | 42 | ++ | 6/6 | 7370.42 ± 9178.25 | 1:256 | ++ | 3/3 | 2.27 ± 0.73 | 1:512 | ++ | 0/3 | 0.01 | |||||||||
3.3 | 44 | ++ | 6/6 | 2307.22 ± 1754.57 | 1:256 | ++ | 3/3 | 39.60 ± 43.86 | 1:128 | ++ | 0/3 | 0.01 | |||||||||
3.4 | NA | 5/6 | 62.07 ± 95.88 | 1:400 | ++ | 2/3 | 3.07 ± 2.72 | 1:400 | ++ | 2/3 | 3.33 ± 3.52 | 1:200 | + | 0/3 | 0.01 | 1:256 | + | 1/3 | 0.67 ± 1.15 | ||
3.5 | NA | 6/6 | 823.41 ± 714.63 | 1:1024 | ++ α | 3/3 | 7.74 ± 3.20 | 1:256 | + | 3/3 | 6.54 ± 0.30 | 1:64 | ++ | 3/3 | 15.91 ± 7.92 | ||||||
Group 4 (102 tachyzoites, IV) | 4.1 | NA | 6/6 | 1334.88 ± 561.06γ | 1:400 | + | 3/3 | 12.44 ± 7.18 | 1:64 | * | 3/3 | 37.24 ± 17.02 | |||||||||
4.2 | NA | 6/6 | 6160.29 ± 4646.09γ | 1:400 | + | 1/3 | 1.22 ± 2.11 | ||||||||||||||
4.3 | 35 | ++ | 6/6 | 2101.24 ± 1290.86γ | 1:512 | ++ | 3/3 | 23.03 ± 3.12 | 1:1024 | ++ | 3/3 | 14.35 ± 12.77 | |||||||||
4.4 | 42 | ++ | 6/6 | 3226.22 ± 2350.13γ | 1:512 | ++ | 3/3 | 45.58 ± 22.09 | 1:128 | ++ | 3/3 | 20.07 ± 13.80 | |||||||||
Group 5 (104 tachyzoites, SC) | 5.1 | NA | 6/6 | 65.40 ± 27.49γ | 1:800 | + | 3/3 | 3.87 ± 1.85 | 1:200 | + | 1/3 | 0.65 ± 1.12 | 1:800 | + | 3/3 | 7.22 ± 5.87 | |||||
5.2 | 35 | + | 6/6 | 707.20 ± 614.80 | 1:512 | ++ | 3/3 | 16.07 ± 2.49 | 1:256 | ++ α | 2/3 | 2.62 ± 2.31 | |||||||||
5.3 | 32 | +++ | 6/6 | 38 294.17 ± 23 816.82 | 1:512 | +++ | 3/3 | 114.35 ± 34.34 | |||||||||||||
5.4 | 37 | + | 6/6 | 781.48 ± 444.87γ | 1:128 | + | 3/3 | 6.32 ± 5.64 | 1:256 | ++ | 3/3 | 2.81 ± 1.58 | |||||||||
Group 6 (uninfected) | 6.1 | NA | 0/6 | 0.01 | – | – | 0/3 | 0.01 | – | – | 0/3 | 0.01 | |||||||||
6.2 | NA | 0/6 | 0.01 | – | – | 0/3 | 0.01 | – | – | 0/3 | 0.01 | ||||||||||
6.3 | NA | 0/6 | 0.01 | – | – | 0/3 | 0.01 |