Figure 3From: Elevated faecal ovotransferrin concentrations are indicative for intestinal barrier failure in broiler chickensOvotransferrin stability in faeces from birds. The ovotransferrin stability in the faeces was assessed by spiking two independent faeces pools from healthy birds with ovotransferrin at a final concentration of 200 µg/g. A The spiked samples were aliquoted and incubated at room temperature for 0, 1, 2, 6 or 24 h after which the ovotransferrin concentration was measured by ELISA. B The ovotransferrin stability in the faeces with (black) or without (grey) the addition of protease inhibitors was assessed after 0, 2 or 24 h incubation at room temperature. The % ovotransferrin recovery was calculated relative to the 0 h timepoint.Back to article page