Figure 7

Binding and cellular localization of rTsSP with IEC by IFT (×200). The IEC was incubated with rTsSP, IIL ES proteins or PBS at 37 °C for 2 h. The C2C12 cell was also incubated with rTsSP at 37 °C for 2 h. Following washing, the IEC and C2C12 was incubated with anti-rTsSP serum, infection serum or pre-immune serum, and then colored with FITC-labeled goat anti-mouse IgG. Cell nuclei were stained with propidium iodide (PI) as red color.