Figure 9

The in vitro inhibition of T. spiralis invasion of IEC by anti-rTsSP serum. Left: upon IIL addition to an IEC monolayer, the invaded and non-invaded larvae in monolayer (A, ×100). B, C Invaded larva in monolayer (×200). D (×200) Non-invaded and suspended larva in culture media (×200). Right: inhibition of larval invasion of IEC through various dilutions of anti-rTsSP serum. Infection serum (IS, 1:50 dilution) and pre-immune serum (PI, 1:50) were respectively utilized as a positive and negative control. The results are expressed as the percentages of the larvae invading the monolayer and they are shown as the mean ± SD of three independent experiments. Asterisks indicate remarkable differences (P < 0.01) relative to pre-immune serum.