Interaction between vimentin and a series of ANXA2-Flag mutants. A Schematic representation of the predicted protein domains of ANXA2. The full-length ANXA2 protein and five truncated ANXA2 mutants were examined in this study. B 293T cells co-transfected with pVIM-HA and plasmids expressing the ANXA2 mutants were collected at 48 h and subjected to co-IP assay using mouse anti-HA affinity gel. The precipitated proteins were analyzed by Western blotting using antibodies against Flag or HA.