Figure 8

Immunofluorescent assay of rTsgal binding to IECs (× 200). The IECs were pre-incubated with rTsgal or PBS, the C2C12 s were pre-incubated with rTsgal at 37 °C for 2 h. Then, IECs were probed by anti-rTsgal serum, infection serum or normal serum, finally stained by using anti-mouse IgG-FITC conjugate, and the cell nuclei were dyed red with propidium iodide.