Figure 2From: The effects of zinc amino acid complex supplementation on the porcine host response to Lawsonia intracellularis infectionEvaluation of immunohistochemistry and microscopic lesions. At 14, 21 and 28 dpi immunohistochemistry (IHC) was performed to semi-quantify L. intracellularis in tissue and hematoxylin and eosin (HE) stain was performed to evaluate microscopic lesions. IHC scores used were of 0: absence of antigen in tissue; 1: 0–25%, 2: 25–50%; 3: 50–75%; 4: 75–100% of enteric crypts containing L. intracellularis antigen. HE scores used were of 1: focal lesions; 2: multifocal lesions; 3: diffuse lesions. A IHC score per time point after infection. B Percent of animals with microscopic lesions evaluated by HE. C HE score per time point after infection. Different letters indicate statistical significance (p < 0.05), error bars represent the standard error.Back to article page